Project Stages

Stage 1 – Optimization of SERS Detection of Extracellular DNA

• Synthesis of DNA fragments simulating extracellular DNA
• Identification of the optimal SERS substrate for detecting DNA fragments simulating extracellular DNA
• Optimization of SERS detection of extracellular DNA
• Extraction of extracellular DNA from the growth medium of tumoral and non-tumoral cell cultures

Stage 2 – Detection of the Type and Degree of Extracellular DNA Methylation via SERS Spectroscopy

• Acquisition of SERS spectra of extracellular DNA
• Multivariate statistical analysis of SERS spectra to differentiate tumoral extracellular DNA
• Multivariate statistical analysis of SERS spectra for quantification of extracellular DNA methylation levels